Acquisition

SleepSign-Rec

SleepSign-Rec is a dedicated aquisition system for SleepSign.
It records analog signals such as EEG and EMG, and can also perform simultaneous video recording.
Compared to the previous version, it enables longer recording periods.
Additionally, by using the optional “Sleep Stage Closed-Loop Function,” real-time scoring and signal output to external devices based on scoring results are possible.

*SleepSign-Rec is the successor to VitalRecorder and SleepSign-Recorder.

Feature

Feature image
  • Digitizes analog signals such as EEG and EMG for each chamber and saves them to data files (*.kcd).
  • A single PC can record up to 4 chambers.
  • Video recording is possible simultaneously with EEG, EMG, and other signals.
  • The Sleep Stage Closed-Loop (optional) enables real-time scoring and signal output to external devices based on scoring results.
  • SleepSign-Rec is a program dedicated to data recording only. Post-recording analysis is performed using SleepSign, the sleep analysis research program.
  • Data recorded with SleepSign-Rec is analyzed by SleepSign.

Video Recording / Playback

Video Recording / Playback image

[Video Recording]

Digital video files acquired with third-party systems such as Microsoft Windows Media Encoder can be imported.
By applying a multiplexer, up to 4 video inputs can be recorded.

[Video Playback]

  • Video data can be reviewed together with physiological data such as EEG and EMG.
  • Event markers can be inserted while observing the video. This allows abnormal animal behavior to be highlighted and quantified.
  • SleepSign window images can be saved as AVI files. This makes it easy to create visual aids for presentations.

Hardware

SleepSign data acquisition program and related hardware enable you to acquire data from analog output of your amplifier and store as digital data in a PC.

Using your acquisition system

By connecting to the BNC Connectors terminal box, you can record the analog signals output from your acquisition system.

Hardware image

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To record analog signals, the following products are required.

No. Product Name Type Num Maker
1 Analog I/O PCI Express Card AIO-163202UG-PE 1 CONTEC
2 BNC Connectors terminal box for Analog I/O Card ATP-16E 1 CONTEC
3 Buffer Amplifier Function Extension Box for analog input card ATBA-16E 1 CONTEC
4 Shield Cable 5m PCB37PS-0.5P 2 CONTEC
5 Conversion shield cable 16ch DT-G2E-16 1 CONTEC

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Please procure the products listed in the configuration table locally.

Biotex Amplifiers

Biotex amplifiers are engineered specifically for sleep research, offering unparalleled performance and reliability in biological signal acquisition.

Traditionally, measuring biological signals such as EEG and EMG in experimental animals has been a complex and time-consuming process. Researchers were required to select, configure, and customize equipment combinations tailored to their specific measurement needs, while also establishing a noise-free recording environment—placing a substantial burden on valuable research time.
To address these challenges, we’ve developed the ideal solution: a streamlined recording system specifically engineered for sleep measurement. Featuring only the essential signals—EEG, EMG, and locomotor
activity—our system eliminates the need for complex amplifier configuration while incorporating advanced noise reduction technology.
This is the Biotex measurement device—designed to let you focus on your research, not your equipment setup.

*Currently, Biotex products are sold only in Japan and China.

Biotex Amplifiers image

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  • Electrode

    Electrodes are available for purchase for both mice and rats.
    Since rats are particularly stronger than mice, the rat electrodes employ a special design that is resistant to detachment by using a clutch to assist the connection strength and utilizing a SATA connector.

    Biotex Amplifiers image
  • Junction Cable

    Connect the Slip ring with the Electrode that mounted on the mouse head.
    The cable has a soft material, so it does not limit the animal behavior.
    The cable material can be selected from Nomal type or Higrade type, and the cable
    length also can be selected from 30cm or 40cm.

    Junction Cable image
  • Slip Ring

    Slip Ring rotates with the movement of the mouse or rat and prevents the cable
    from twisting.
    There are two types available: with hole and without hole type.

    • Without hole typeSL-006(22): This is the standard type. Please use this for recording normal EEG and EMG.
    • With hole typeSL-006(32) H: This type has a hole for passing wires, which is necessary for drug administration, etc. Please use this type if you want to perform continuous drug administration or stimulation using fibers, etc., on the animal during recording.
    Slip Ring image
  • Head Amplifier

    Head Amplifier is an EEG and EMG amplifier for measuring EEG and EMG of small
    animals. For models with Locomotor, the animal activity is also measured with a 3D
    infrared pyroelectric sensor.

    Head Amplifier image
  • Interface cable

    Connect the Interface Box and Head Amplifier.

    Interface cable image
  • InterFace Box

    InterFace Box supplies power to four EEG/EMG amplifiers (head amplifiers) and relays signals to the A/D converter (CONTEC/AIO-161601UE3-PE). This device has a connector for direct connection to the A/D converter. One unit can supply power to up to four head amplifiers.

    Interface cable image
  • Calibrator

    Output ±100㎶signal for calibration of Amplifier.

    Calibrator image
  • Cage for Mouse

    A mouse breeding cage for experiments.

    InterFace Box image

Closed Loop Option

ClosedLoop-option is a function that estimates sleep stages in real time from EEG and EMG signals, while simultaneously outputting a signal when the subject transitions to a specified sleep stage.

Feature

  • Controls optogenetics and various other external devices.
  • Output feedback stimuli are non-isolated open-collector outputs.
  • Up to four subjects can be individually controlled from a single PC.
Feature image

To output a trigger signal, the following product is required.

Product Name Type Num Maker
Digital output USB I/O unit 16ch DO-16TY2-USB 1 CONTEC
BNC/M TO OPEN BNCPR-58AU0100 1-4 -

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Specifications

Supported OS and PC : The desktop PC for recording must be a model specified by our company or one with equivalent specifications. Please contact your nearest distributor or our company.
Input Chambers/Channels Up to 4 chambers, up to 8 channels per chamber (when analog input boards are added)
Sampling Rate 128 Hz, 200 Hz, 256 Hz, 500 Hz, 512 Hz, 1000 Hz, 1024 Hz, 2000 Hz, 2048 Hz
Data Acquisition Manual start/stop, timer scheduling, all chambers simultaneously, per chamber
Data Display Functions Raw data: Display all chambers, display per chamber
Data Storage Raw data file (*.KCD) per chamber
Video Composition Saves every 10 minutes per file.
Data Display Function Raw Data: All chambers displayed, video
Data Storage Video file (*.avi)
Input Video Format NTSC
Resolution NTSC (640×480)
Analog Input RCA 1 channel (composite) ※ Audio cannot be recorded
Data Display Function Video data (Up to 4 fish videos can be displayed on one screen using a screen splitter) ※ Video recording cannot be performed per chamber.
Filter FIR, IIR, Low-Pass, High-Pass, Band-Pass, Band-Stop
Cutoff frequencies can be freely specified.
Sleep Stage Estimation Same algorithm as SleepSign can be used.
  • Determination Parameters: Delta waves, Theta waves, EMG,
    Locomotor, etc.
  • Detection Methods: FFT-based content/ratio, integral values,
    cross-count, etc.
  • Logic: if-then (conditional branching) method
Data Output Format
  • Non-isolated open collector output
    Selectable as open or closed active according to external device specifications
Output Channels 1 channel per chamber, maximum 4 channels
Output Connector Terminal connector (screw type)
※Can be converted to BNC (male) using the separately sold BNC male to lead wire splitter.
Start and End of Output Output begins when transitioning to a specified stage and ends when transitioning to a non-specified stage.
*Actual output start and end times are delayed by the epoch seconds.
Data Display Functionality
  • Raw Data: Display for all chambers
  • Trend Data: Sleep stage estimation results, sleep stage estimation parameters
  • During feedback stimulus output, a portion of the screen
    illuminates red.
  • Events indicating output start or end are displayed on the waveform.
Data Saving Per chamber: Raw data file (*.KCD), Judgment result file (*.RAF), Log file (*.log)
※ Feedback stimulus output start and end times are recorded in the log file. They are also recorded as events within the raw data file.
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